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The Single Wire Myograph System - 320A is ideal for studying a single vessel with a diameter of 30μm - 3mm. The vessel is mounted as a ring preparation by threading it over two parallel stainless steel wires and securing the wires to two mounting “jaws.” One support is attached to a precision micrometer, allowing manual control of vessel circumference and stretch. The other support is attached to a force transducer for measurements of force/tension development.

The preparation is mounted in a heated 10ml acid-resistant stainless steel chamber, which can be covered with a lid featuring ports for rapid suction/draining, refilling, and bubbling of oxygen. The base of the chamber contains a window allowing morphological observation or fluorescence measurements on an inverted microscope.

Typically, the preparation is kept in the heated vessel chamber in a physiological salt solution at 37°C, bubbled with oxygen, where the vessels remain viable for up to 12 hours.

Following mounting and equilibration, the passive length-tension relationship of the vessel is determined. During the actual experiment, the circumference of the vessel is kept constant. Compounds can be added directly to the chamber, and the vessel's contractility and reactivity are measured under isometric conditions.

An electronic valve can be added to the system for easy control and emptying of the chamber as an option.

​The Wire Interface with a touchscreen makes it easy to set up and use. MyoDAQ acquisition software is used to collect data. The data analysis is unique in being able to add multiple data selections throughout the course of the experiment in single or numerous channels. Data can be exported via a selection of the trace or the entire data file for external charting or custom analysis.
Related Products ​​​​
  • Computer Package 
  • Data Acquisition
  • Dissection Stereo Microscope
  • Dissection Tool Kit
  • Extended warranty years *1
  • Electronic Valve for buffer drainage
  • Field Stimulation Electrodes
  • Gas supply manifold - 4-channel 
  • Light Source LED​
  • Open Heating Bath Circulator 
  • pH electrode  & pH Meter
  • Service & Maintenance Agreement​
  • ​Stimulator
  • Tungsten Wire (25µm, 15µm, or 10μm)
  • Vacuum Package​

Wire Myograph Chamber

​# Ring-mounted segments - 30µm to 3mm
# Fine scale micrometer for accurate tension control
# Force range ±200mN 
# Acid resistent stainless steel chamber 
# Optical base window for imaging

Interface Unit

# Direct data streaming
# Integrated heat control
# Intregreted calibration procedure
# ​Intuitive touch-screen menus
# Compact design / small footprint
  • Technical Data
  • Guides & Manuals
  • Related Products
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Chamber:
​​Chamber volume (Min/Max):
Chamber(s):
​Chamber sharpe:
Chamber material:
Vessel size:
Vessel normalization:
Micrometer resolution:
Mounting type:

​4.2 ml / 10 ml
1
Square
Acid-resistant stainless steel
>30 µm
Manually
0.01 mm
Jaws 
Temparature:
​Range:
Resolution:
Stability:
Heating:
​​
​15.0 to 50.0 °C
0.1 °C
±0.2 °C
​Yes
Transducer:
​Output reading:
Range:
​Frequency range:
Resolution:
Force calibration:

​mN
±200 mN
0-20 Hz
0.01 mN
Yes
Output:
​Data communication:
Analogue output channels:
Analogue output range:

​USB 2.0
2
​±2.5 V
Need To Have:
Data Acquisition – MyoDAQ (#300671)
Computer Package (Desktop) (#101022)
Dissection Stereo Microscope - (#400484)
Light Source LED- gooseneck guide (#400794)
Dissection Tool Kit (#101017)
Open Heating Bath Circulator- Water bath 110V (#400738), 220V (#400740)
Vacuum Package - 110V (#101018), 220V (#101052)
Nice To Have:
Force transducer- isometric (#300172)
Installation Kit (#300639)
Gas supply manifold- 4-channel (#300295)
pH electrode (#300370)
pH Meter License (#101040)
Tungsten Wire - 25μm (#300096) 15μm (#300134), 10μm (#300135)
Service & Maintenance Agreement (5yr) (#101054) Extended warranty +3 years (#101034)
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