Reduce workload
Automate filling steps that would otherwise require repeated manual pipetting during washing and buffer-replacement protocols.
Buffer Filler System – 625FS
Automate buffer filling and repetitive wash routines
The DMT Buffer Filler System – 625FS is an add-on for DMT four-channel systems. It provides controlled filling of one selected chamber or all four chambers, supports repeatable buffer volumes and simplifies protocols that require repeated emptying, washing and refilling.
4 channels
Compatible with DMT four-channel systems.
2–8 mL
User-selectable low and high filling volumes.
1–10 cycles
Configurable automated empty-and-fill wash routine.
Automatic four-channel buffer filling
Why choose 625FS
Reduce repetitive handling during four-channel experiments
Repeated buffer changes can require many manual pipetting steps and repeated handling around mounted tissue and force transducers. The 625FS helps standardize this part of the workflow across compatible DMT four-channel myograph, organ bath and muscle systems.
Consistent filling volumes
Apply calibrated, repeatable volumes across chambers to reduce user-dependent variation during wash-out routines.
Protect the preparation
Reduce physical handling close to mounted tissue and sensitive force transducers during repeated buffer changes.
Simplify cleaning
Use automated filling and washing together with the system vacuum function to support faster cleaning after experiments.
Daily laboratory workflow
Automate one of the most repetitive steps in ex vivo experiments
Concentration-response, wash-out and tissue-preparation protocols may require each chamber to be emptied and refilled many times. The 625FS delivers buffer through dedicated tubing to each chamber while the compatible DMT interface controls filling and wash settings.
Individual control
Select and fill one chamber independently when required by the
protocol.
Four-channel filling
Fill all four chambers from a single interface command.
Volume settings
Store low and high filling volumes between 2 and 8 mL.
Temperature support
Built-in heating helps maintain buffer temperature during
delivery from a preheated reservoir.
Core functions
Filling, washing and workload relief in one add-on system
The 625FS is built around practical requirements in repeated four-channel washing and buffer-replacement workflows.
Single-chamber or all-chamber filling
Fill one chamber independently or deliver buffer to all four chambers from the compatible interface workflow.
Selectable low and high volumes
Configure separate low- and high-volume settings between 2 and 8 mL to support final filling and multi-cycle wash routines.
Automated wash routines
Set between one and ten empty-and-fill cycles for protocols that require repeated wash-out between experimental interventions.
Calibrated chamber delivery
Calibrate the delivered volume for each channel to support accurate and consistent filling after installation or relocation.
Adjustable temperature setpoint
Set the internal filler temperature between 20 and 45 °C to help maintain the required chamber temperature during buffer delivery.
Cleaning support
Run the filling and washing pathway with distilled water after use to help remove buffer deposits from the system and tubing.
Operating principle
A controlled four-step buffer replacement workflow
Once installed and activated on a compatible interface, the system coordinates reservoir delivery, chamber filling and the existing vacuum drainage pathway.
Prepare the reservoir
Fill the reservoir bottle with preheated buffer and position it at the recommended operating level.
Select the chamber
Choose an individual chamber or all four chambers from the interface controls.
Drain and refill
Use the vacuum pathway to empty the chambers and the 625FS to deliver the selected buffer volume.
Repeat automatically
Run the selected number of wash cycles and finish with the stored final filling volume.
System compatibility
Compatible with DMT four-channel systems
The Buffer Filler System is designed for DMT four-channel platforms, including wire myographs, organ bath systems and muscle myograph systems. It can be supplied with a new system or evaluated for retrofit to an existing compatible installation.
For retrofit installations, provide the DMT system model and
serial number. DMT can confirm the interface, firmware, connection
and mechanical configuration before quotation.
Multi Myograph System – 620M
Four-channel wire-myograph platform for parallel vascular and
smooth-muscle experiments.
Automated Multi Myograph – 630MA
Four-channel automated wire-myograph platform with integrated
normalization and compatible buffer-exchange workflows.
Multi Organ Bath System – 820MO
Four-channel organ bath platform for isolated tissue and organ
preparations requiring repeated buffer changes and wash-out
routines.
Multi Chamber Myograph System – 840MD
Four-channel muscle and tissue myograph platform compatible with
automated chamber filling and washing workflows.
Other DMT four-channel systems
Compatibility is based on the four-channel system architecture,
interface, firmware, connection and mechanical configuration.
Interface firmware
The interface must use compatible firmware. Confirm the current
version with DMT before retrofit.
Interface connection
A compatible RS232 female connection is required on the DMT
interface unit.
Mechanical fit
Confirm that the filler unit and tubing can be mounted and
aligned correctly with the intended four-channel system.
Technical data
Key 625FS specifications
Core filling, temperature, washing, electrical and physical specifications for evaluating the Buffer Filler System.
Product designation: Filler System – 625FS/3. Part number: 101057.
Intended for ex vivo laboratory applications only.
System
- Number of channels
- 4
- Primary use
- Automatic chamber filling and wash-out
- System compatibility
- DMT four-channel systems
- Tubing length
- Maximum 100 cm
- Tubing dimensions
- 6 × 3 mm
Filling
- Low-volume range
- 2–8 mL
- High-volume range
- 2–8 mL
- Volume accuracy
- 0.2 mL
- Filling speed
- 20 mL/min
Temperature
- Temperature setpoint
- 20–45 °C
- Temperature resolution
- 0.1 °C
- Temperature stability
- ±0.3 °C
Washing
- Automated washing routine
- 1–10 empty/fill cycles
- Chamber selection
- Individual chamber or all four
- Valve delay
- User adjustable
Electrical
- Operating voltage
- 100–240 VAC automatic
- Power adapter output
- 24 VDC ±10%
- Maximum current
- 3.3 A
Environment
- Weight
- 2.1 kg
- Operating temperature
- 15–30 °C
- Storage temperature
- 4–70 °C
- Humidity
- 20–80% RH, non-condensing
Resources
Setup guidance and compatible four-channel systems
Use the confirmed DMT resources below when evaluating compatibility, installing the 625FS or planning a four-channel laboratory workflow.
625FS quick guide
Installation, activation, calibration, volume settings, operation and cleaning guidance.
→Multi Myograph System – 620M
Review the four-channel wire-myograph workflow compatible with the Buffer Filler.
→Automated Multi Myograph – 630MA
Review the automated four-channel platform and normalization workflow.
→Multi Organ Bath System – 820MO
Review the four-channel organ bath platform compatible with automated filling and washing.
→Wire myograph systems
Review DMT wire-myograph systems and four-channel configurations.
→Organ bath systems
Review DMT organ bath systems for isolated tissue and organ studies.
→Muscle myograph systems
Review DMT muscle and tissue systems, including four-channel workflows such as the 840MD.
→Technical support
Request assistance with retrofit compatibility, setup, calibration or operation.
→Compatibility guidance
Adding automatic buffer filling to a DMT four-channel system?
Share the DMT system model, serial number, interface firmware version, available connection, preferred chamber volumes and washing requirements. DMT can confirm compatibility with systems such as the 620M, 630MA, 820MO and 840MD and define the appropriate 625FS configuration.