Four-channel parallel design
Four chambers allow vessels or tissue rings to be studied simultaneously, supporting repetitive concentration-response protocols and treatment-control comparisons.
Multi Myograph System – 620M
Four-channel wire myography for parallel vascular reactivity studies
The DMT 620M is a robust four-channel wire myograph for in vitro studies of vascular structure and function. It supports isometric contraction and relaxation studies using small arteries, larger vessels and a broad range of tissue ring preparations.
Measurement
Isometric force in four independent preparations.
Preparations
Small arteries, larger vessels and tissue rings.
Acquisition
MyoDAQ, direct LabChart 8 USB streaming or analogue outputs.
4
independent experimental chambers
Why choose 620M
A trusted platform for reproducible vascular pharmacology and physiology
The 620M is designed for laboratories that need a flexible, durable and practical wire myograph for concentration-response curves, compound testing and parallel studies of individual vessel segments.
Small and larger ring preparations
Jaw and pin mounts support work with small vessels as well as larger ring segments, providing flexibility across different tissues and experimental protocols.
Controlled chamber conditions
Built-in heating, individual gas inflow and chamber drainage help maintain stable experimental conditions across all four channels.
Manual normalization workflow
Passive length-tension relationships can be determined following mounting and equilibration to improve consistency between vessel segments and experiments.
Direct data communication
The interface provides calibration functions, analogue output channels and USB communication for compatible DMT acquisition workflows.
Expandable laboratory setup
Multiple 620M systems can be arranged side by side, particularly when combined with the Buffer Filler System – 625FS for expanded experimental capacity.
Measurement and experimental fit
What the 620M measures—and when to choose another method
The 620M is designed for experiments in which isometric force is the principal endpoint. Separating direct measurements from calculated outcomes helps researchers select the correct platform and analysis workflow.
Directly measured with the 620M
Each chamber records the force generated by a mounted vessel or tissue preparation while its length is held constant.
- Isometric force in mN or g
- Contraction and relaxation over time
- Responses to pharmacological or physiological stimulation
- Four preparations recorded simultaneously
Commonly calculated during analysis
Recorded force traces can be used to derive commonly reported vascular reactivity outcomes.
- Maximum response and percentage relaxation
- Emax, EC50 or pEC50
- Area under the curve
- Responses normalized to a reference contraction
What the 620M does not directly measure
The 620M does not directly measure vessel diameter, intraluminal pressure or luminal flow. These endpoints normally require a pressure-myograph system.
Best suited for
- Vascular contraction and relaxation studies
- Endothelial-function assessment
- Concentration-response experiments
- Comparative pharmacology and compound testing
- Mouse and rat vessel preparations
- Four simultaneous, independently treated preparations
System overview
Classic wire myography with four individually controlled chambers
Each 620M myograph unit has a centrally positioned, acid-resistant stainless-steel chamber. Tissue supports, either jaws or pins, are located inside the chamber with one side attached to the force transducer and the other to a precision micrometer.
During experiments, compounds can be added directly to the individual baths while contraction and relaxation are measured under isometric conditions.
Four independent chambers
Run parallel vessel or tissue ring studies in separated baths.
Jaw and pin mounting
Select the mounting approach according to vessel size, tissue
type and protocol.
Integrated control
Maintain chamber temperature, gassing and drainage through the
shared interface.
Manual normalization
Standardize passive length-tension conditions before active
experimental protocols.
Applications
Vascular physiology and pharmacological tissue reactivity
The 620M is suited to studies of vascular contraction, relaxation and reactivity in small arteries, larger vessels and other ring-mounted preparations.
Its four-chamber format supports concentration-response protocols, compound testing and experiments where individual preparations need to be studied in separate baths.
Hypertension
Atherosclerosis
Diabetes
Pre-eclampsia
COPD and asthma
Gastrointestinal research
Bladder research
01
Endothelial-function studies
Evaluate endothelium-dependent and endothelium-independent relaxation following precontraction of the vessel preparation.
02
Vascular contraction and relaxation
Measure active force development and relaxation under controlled isometric conditions.
03
Concentration-response studies
Run repeated pharmacological protocols across four independent experimental chambers.
04
Small-artery research
Support ring-mounted small-vessel preparations using the appropriate jaw mounting configuration.
05
Larger vessels and tissue rings
Study aorta, carotid artery, trachea, intestine, bladder and related ring-mounted tissue preparations.
Selected scientific publications
Research applications demonstrated with the DMT 620M
The following peer-reviewed studies illustrate how researchers have used the DMT 620M for vascular reactivity, endothelial-function and smooth-muscle investigations across different tissues and disease models.
A Novel Role for the Small Molecule Cinnamaldehyde in Protecting Against P. gingivalis-Induced Endothelial Dysfunction in Mice
Second-order mesenteric arteries were mounted in a DMT 620M to assess contractile responses and endothelium-dependent relaxation following P. gingivalis exposure and cinnamaldehyde treatment.
View publicationHeavy Alcohol Consumption Attenuates Human Mesenteric Artery Responsiveness to Sigma Receptor-1 Ligands
Human mesenteric artery rings from organ donors were studied using a DMT 620M to examine sigma-receptor modulation of phenylephrine-induced contraction.
View publicationEndothelial Cells Increase Mesenchymal Stem Cell Differentiation in Scaffold-Free 3D Vascular Tissue
Scaffold-free vascular tissue rings were mounted in a DMT 620M to quantify endothelin-1-mediated contraction and evaluate functional smooth-muscle differentiation.
View publicationGut Microbial Metabolite Imidazole Propionate Impairs Endothelial Cell Function and Promotes the Development of Atherosclerosis
Aortic segments were studied using a DMT 620M as part of a translational investigation into endothelial dysfunction, vascular reactivity and atherosclerosis.
View publicationSoftware and data acquisition
Three flexible ways to acquire 620M force traces
Select the acquisition route that best matches the laboratory's software, existing hardware and preferred analysis workflow.
DMT MyoDAQ through USB
Connect the 620M to a compatible computer through USB and acquire all four force channels in DMT MyoDAQ for recording, display and export.
Learn about MyoDAQDirect USB streaming to LabChart 8
Stream the four force channels directly into LabChart 8 through USB using the appropriate DMT integration and a compatible LabChart configuration.
Four analogue outputs
Four independent ±2.5 V analogue outputs allow the force signals to be recorded with compatible external data-acquisition hardware.
Configuration note
LabChart, PowerLab and other third-party hardware or software are supplied separately unless specifically included in the quotation. DMT can confirm the appropriate interface for the intended acquisition route.
System components
Integrated hardware for chamber control and force measurement
The 620M workflow combines four myograph chamber units with the shared Multi Interface for environmental control, calibration and communication with the selected data-acquisition setup.
620M chamber unit
Supports ring-mounted vessel segments using jaw or pin mounting, with precision mechanical adjustment and sensitive isometric force measurement.
Multi Interface
Provides shared control of heating, gassing, drainage, selectable force range, calibration and direct data communication across all four channels.
Product videos
Explore the 620M setup and operating principles
DMT product and how-to videos provide practical guidance for system setup, calibration, mounting, normalization and maintenance.
Introduction
View product videos
System introduction
Overview of the 620M and its principal components.
Setup
View product videos
System setup
Guidance for preparing the 620M for experimental use.
Calibration
View product videos
Force calibration
Calibration guidance for consistent force measurement.
Mounting
View how-to videos
Jaw and pin workflows
Mount changes, alignment and vessel-mounting guidance.
Normalization
View how-to videos
Normalization guidance
Manual normalization and micrometer-adjustment guidance.
Maintenance
View how-to videos
Cleaning and service
Routine cleaning, maintenance and transducer guidance.
Technical data
Key 620M specifications
Core chamber, temperature, transducer and output specifications for planning a complete 620M installation.
The 620M uses manual normalization. Vessel handling, mounting
quality, equilibration time and consistent normalization procedures
remain important for reproducible vascular reactivity data.
Chamber
- Number of chambers
- 4
- Minimum volume, jaw mount
- 3.8 ml
- Minimum volume, pin mount
- 2.0 ml
- Chamber material
- Acid-resistant stainless steel
- Vessel size, jaw mount
- >30 µm
- Vessel size, pin mount
- >450 µm
- Normalization
- Manual
- Micrometer resolution
- 0.01 mm
- Mounting type
- Jaws and pins
Temperature
- Temperature range
- 15.0 to 50.0 °C
- Temperature resolution
- 0.1 °C
- Temperature stability
- ±0.2 °C
- Heating
- Yes
Transducer
- Output reading
- mN or g
- Selectable force range
- ±200 / ±400 / ±800 / ±1600 mN
- Force resolution
- 0.01 mN
- Force calibration
- Yes
Data output
- Digital communication
- USB 2.0
- Analogue output channels
- 4
- Analogue output range
- ±2.5 V
Configuration guidance
Plan a complete and clearly specified 620M workflow
Final contents depend on the selected mounting configuration and quotation. Separate standard system elements, optional DMT equipment and laboratory-supplied infrastructure when planning the installation.
620M system configuration
Four myograph chamber units, the Multi Interface, selected mounting supports, system cabling and standard accessories are specified in the quotation.
Optional DMT equipment
Options may include the Buffer Filler System – 625FS, alternative jaws and pins, volume-reducing inserts, tungsten wire, spare transducers, installation, training, service agreements and extended warranty.
Normally supplied by the laboratory
Typical supporting infrastructure includes a compatible computer, dissection microscope and tools, gas supply, vacuum source, buffer and reagents, and any third-party acquisition hardware selected for the workflow.
Frequently asked questions
Common questions about the 620M
Can the 620M be used with mouse mesenteric arteries?
Yes. Small arteries can be mounted using the appropriate jaw configuration, provided the vessel dimensions and handling requirements are compatible with the selected setup.
Can the 620M be used with rat aortic rings?
Yes. Larger vessel rings can be mounted using pin supports selected for the preparation size.
Can all four chambers run different treatments?
Yes. The chambers are separate, allowing different compounds, concentrations or experimental conditions to be applied independently.
Does the 620M perform automatic normalization?
No. Normalization is performed manually by adjusting the micrometer according to the selected procedure. Laboratories requiring automated normalization should consider the DMT 630MA.
Can the 620M stream directly to LabChart 8?
Yes. The system supports direct streaming of all four force channels into LabChart 8 through USB using the appropriate DMT integration and a compatible LabChart configuration. The signals can alternatively be recorded through the analogue outputs.
Is a PowerLab required?
Not when the validated direct USB-to-LabChart workflow is used. A PowerLab or other compatible acquisition system may be used when recording from the analogue outputs.
Is the 625FS Buffer Filler compatible?
Yes. The 625FS is compatible with the 620M and can support buffer handling in expanded multichannel workflows.
Does the 620M measure vessel diameter?
No. The 620M measures isometric force. A pressure myograph is normally used when vessel diameter is the primary endpoint.
Resources
620M documentation and supporting guidance
Use the product data sheet, manuals, normalization guidance and software resources when evaluating, configuring or operating the 620M.
620M product data sheet
Current product overview, applications and technical specifications.
→620M user manual – v3.3
Detailed system setup, calibration and maintenance information.
→620M user guide – v3.0
Practical reference for routine preparation and system use.
→Normalization guide – v2.0
DMT guidance for wire-myograph normalization workflows.
→Software downloads
Find DMT acquisition software, installers and related tools.
→Guides and manuals
Browse additional DMT product and application documentation.
→Wire myograph systems
Compare DMT wire-myograph platforms and measurement workflows.
→Training and installation
Support laboratory onboarding and consistent system operation.
→
Technical review
Product information reviewed by DMT Product Management and Application Support.
Last technical review: July 2026.
Product information reviewed by DMT Product Management and Application Support.
Last technical review: July 2026.