Pulsation simulation
Simulate pulsations between 50 and 600 BPM with a pressure difference of up to 60 mmHg.
Pressure Pulsation Myograph System – 112PP
Pressure, flow and pulsation studies in small isolated vessels
The DMT 112PP is a pressure and pulsation myograph for studying the structure and function of isolated small vessels under near-physiological conditions. It supports constant transmural pressure, non-pulsatile luminal flow workflows and pulsation simulation from 50 to 600 BPM, allowing researchers to investigate pressure, mechanical stretch, vessel diameter and functional vascular responses in one system.
50–600 BPM
Programmable pulsation simulation.
Vessels >40 µm
Cannulated small-vessel preparations.
MyoVIEW ready
Live diameter, pressure and vessel-wall analysis.
112PP
Dynamic pressure and pulsation workflows
Why choose 112PP
Designed for pressure myography when pulsation and mechanical stretch matter
The 112PP extends standard pressure-myograph workflows with dynamic pulsation control, longitudinal force measurement and live vessel-dimension analysis.
Pressure, stretch and diameter
Study small vessels under controlled intravascular pressure while measuring vessel diameter and pressure-dependent responses.
Longitudinal force
Record longitudinal force alongside pressure and diameter for a more complete mechanical characterization.
Low chamber volume
A minimum chamber volume of 3.1 mL helps reduce buffer and reagent consumption.
Integrated system control
Heating, pressure control, reservoir operation and calibration are managed through the Pressure Interface.
MyoVIEW analysis
Track diameter, lumen, wall thickness, pressure and calculated vascular parameters during the experiment.
System overview
Dynamic pressure-myograph studies with pulsatile pressure control
A small isolated vessel is cannulated, aligned and maintained under controlled transmural pressure while vessel diameter and wall parameters are tracked continuously.
The pulsation chambers allow dynamic pressure patterns to be applied in addition to steady-pressure protocols. The system can also support non-pulsatile flow measurement when configured with the DMT FlowMeter – 162FM.
Constant pressure
Study vessels under stable transmural pressure from 0 to
250 mmHg.
Pulsatile protocols
Simulate pulse rates from 50 to 600 BPM.
Pressure amplitude
Apply pulsation pressure differences of up to 60 mmHg.
Longitudinal force
Measure axial vessel force independently from pressure control.
Optional flow measurement
Add FlowMeter – 162FM for non-pulsatile flow measurement from
15 to 4,000 µL/min.
System components
Chamber, interface and complete 112PP system
The 112PP combines a pulsation-specific chamber, Pressure Interface and MyoVIEW-compatible acquisition for controlled pressure, pulsation, force and vessel-dimension studies.
112PP chamber
Acid-resistant chamber for cannulated vessel mounting, pulsation control, in-line pressure measurement, longitudinal force recording and optical visualization.
Pressure Interface
Touch-screen control of pressure, pulsation, heating, calibration and reservoir functions, with USB communication and analogue outputs.
Complete 112PP system
Integrated chamber and interface platform for steady-pressure, pulsatile-pressure and MyoVIEW-compatible small-vessel studies.
Research applications
Myogenic response, pulsation and vascular mechanics
The 112PP is relevant for studies where intact pressurized vessels must be assessed under static pressure, dynamic pressure or non-pulsatile flow conditions.
01
Pulsatile pressure studies
Evaluate vessel responses under programmable dynamic pressure conditions and pulse rates.
02
Myogenic responses
Study pressure-dependent constriction and dilation under controlled steady or pulsatile pressure.
03
Flow-mediated dilation
Investigate flow-dependent endothelial function in non-pulsatile flow workflows.
04
Compliance and stiffness
Apply cyclic pressure changes to assess vascular compliance, mechanical adaptation and stiffness.
05
Pharmacological testing
Apply vasoactive compounds to the superfusate or luminal solution and track changes in vessel diameter and tone.
06
Longitudinal vessel mechanics
Combine axial force measurement with pressure and diameter data during vessel-stretch protocols.
Selected scientific publications
112PP in peer-reviewed research
Selected peer-reviewed research that explicitly reports use of the DMT 112PP Pressure Pulsation Myograph.
High pulse pressure impairs cerebral artery endothelial function in young, but not old, mice
Posterior cerebral arteries were placed in a DMT 112PP pulsatile pressure myograph chamber and exposed ex vivo to static, low-pulse- pressure and high-pulse-pressure conditions to investigate endothelial dilation and vascular stiffness.
View publication
Publications are provided as examples of peer-reviewed research in which
the DMT 112PP was explicitly reported. Inclusion does not imply endorsement
of the authors, institutions, conclusions or experimental methods by DMT.
MyoVIEW workflow
Live diameter tracking and pressure-myograph analysis
MyoVIEW records vessel-wall thickness, vessel and lumen diameter, intravascular pressure and calculated vascular parameters during steady-pressure, flow and pulsation studies.
Live vessel detection
Track vessel diameter and lumen changes continuously during
the experiment.
Multiple scanlines
Use multiple edge-detection zones for robust vessel
measurements.
Protocol markers
Mark compounds, pressure steps, pulse changes and other
interventions during acquisition.
Calculated parameters
Review wall thickness, area, flow, vascular resistance, shear
stress and related outputs.
Technical data
Key 112PP specifications
Core chamber, vessel, pulsation, pressure, force, temperature and communication specifications for evaluating the 112PP.
Flow measurement is available in non-pulsatile modes using
FlowMeter – 162FM. Flow cannot be measured during pulsation, so
the planned experimental protocol should be defined before
quotation.
Chamber
- Number of chambers
- 1
- Minimum chamber volume
- 3.1 mL
- Chamber material
- Acid-resistant stainless steel
- Vessel size
- >40 µm
- Vessel alignment
- X, Y and Z
- Micrometer resolution
- 0.01 mm
- Mounting type
- Glass or stainless-steel cannulas
Pulsation
- Pulse-rate range
- 50–600 BPM
- Pulsation pressure difference
- Up to 60 mmHg
Temperature
- Temperature range
- 15.0–50.0 °C
- Resolution
- 0.1 °C
- Stability
- ±0.2 °C
- Integrated heating
- Yes
Longitudinal force
- Output reading
- mN
- Force range
- ±200 mN
- Force resolution
- 0.01 mN
- Force calibration
- Yes
Pressure
- Output reading
- mmHg
- Pressure range
- 0–250 mmHg
- Pressure stability
- ±0.5 mmHg
- Pressure resolution
- 0.1 mmHg
Reservoir and output
- Reservoir
- Heated, 250 mL
- Pressure circuit
- Closed
- Air inlet
- 1 bar maximum
- Data communication
- USB 2.0
- Analogue outputs
- 4 channels, ±2.5 V
Configuration guidance
Plan the complete pressure and pulsation workflow
The correct 112PP configuration depends on vessel size, microscope and camera setup, pulsatile or steady-pressure protocol, non-pulsatile flow requirements, cannula selection, software and training needs.
Need-to-have equipment
Typical workflows include MyoVIEW acquisition, computer package, inverted microscope, high-speed monochrome camera, stage micrometer, vacuum package and heating bath or circulator.
Flow and mounting options
Confirm glass or stainless-steel cannula sizes, pressure tubing, peristaltic pump requirements and FlowMeter – 162FM for non-pulsatile flow measurement.
Service and training
Installation, vessel-cannulation training, pressure and force calibration guidance, spare pressure transducers and service coverage help support reliable use.
Frequently asked questions
Common questions about the 112PP
What is the Pressure Pulsation Myograph 112PP used for?
The 112PP is used to study small blood vessels under physiologically relevant pressure and pulsatile conditions. It allows researchers to measure vascular function, vessel diameter, compliance, and pressure-dependent responses while simulating the pulsatile environment found in vivo.
What types of blood vessels can be studied with the 112PP?
The 112PP is designed for small arteries and veins greater than 40 µm in diameter. It is ideal for resistance vessel studies where pressure, pulsation, flow, and vascular function are critical to the experiment.
What measurements can the Pressure Pulsation Myograph 112PP perform?
The system measures vessel diameter, wall thickness, intravascular pressure, longitudinal force, myogenic responses, compliance, vascular stiffness, and pressure-dependent changes in vessel function. With MyoVIEW software, researchers can also analyze lumen diameter, shear stress, vascular resistance, and other calculated vascular parameters.
Can the 112PP simulate pulsatile blood flow?
Yes. The 112PP can simulate physiological pulsation rates from 50 to 600 BPM with programmable pressure amplitudes, allowing researchers to investigate how pulsatile pressure influences vascular function, remodeling, compliance, and endothelial responses.
Can the 112PP be used to study myogenic tone and flow-mediated dilation?
Yes. The 112PP is designed for studies of myogenic tone, flow-mediated dilation, endothelial function, mechanical stretch, vascular stiffness, and pharmacological responses under controlled pressure conditions. Optional FlowMeter integration also supports non-pulsatile flow experiments.
What research applications is the 112PP best suited for?
The 112PP is widely used in hypertension, vascular biology, atherosclerosis, endothelial dysfunction, arterial stiffness, cardiovascular disease, pharmacology, and translational vascular research where realistic pulsatile conditions are important for understanding vascular physiology.
What's the difference between the 112PP and the 114P Pressure Myograph?
Both systems study small pressurized blood vessels, but the 112PP adds programmable pulsation simulation and longitudinal force measurement, making it ideal for researchers investigating dynamic vascular mechanics. The 114P is the standard pressure myograph for steady-pressure studies of vascular function and reactivity.
Is the Pressure Pulsation Myograph 112PP the right system for my research?
The 112PP is the best choice if your research requires physiologically relevant pulsatile pressure, vascular mechanics, compliance, or myogenic response studies. If your experiments focus on standard pressure myography without pulsation, the DMT 114P may provide the functionality you need with a simpler workflow.
Resources
112PP documentation and supporting resources
Use the official DMT documentation and related pages when evaluating, configuring or operating the 112PP.
112PP product data sheet
Product overview, applications, specifications and configuration guidance.
→112PP/114P user manual
Detailed setup, pressure circuit, interface and calibration documentation.
→112PP/114P user guide
Practical reference for routine pressure-myograph operation.
→MyoVIEW user manual
Software documentation for vessel tracking and pressure-myograph analysis.
→MyoVIEW software
Live vessel tracking and analysis for pressure-myograph workflows.
→FlowMeter – 162FM
Optional flow measurement for non-pulsatile pressure-myograph protocols.
→Pressure comparison chart
Compare pulsation, flow, force, imaging and vessel-size capability.
→Training and installation
Plan setup, cannulation, calibration and practical user onboarding.
→
Configuration guidance
Planning a pulsatile pressure-myography study?
Share the vessel type and size, pressure range, desired pulse rate and pressure amplitude, steady or pulsatile protocol, longitudinal force requirements, non-pulsatile flow needs, microscope and camera setup and preferred analysis workflow. DMT can help define the appropriate 112PP configuration.