Economical system format
Focus the configuration on pressure, diameter, wall and optional flow endpoints without paying for longitudinal force measurement.
Pressure Myograph System – 114PN
Pressure and flow studies without longitudinal force measurement
The DMT 114PN is a pressure myograph for experiments on pressurized small arteries, veins and other vessels under near-physiological conditions. It is designed for researchers who need static-pressure, vessel-diameter or flow-related studies without longitudinal force measurement, providing a more economical pressure-myograph configuration.
Vessels >40 µm
Cannulated small-vessel preparations.
Pressure and flow
Static pressure with optional luminal flow measurement.
No force transducer
Economical configuration without longitudinal force.
Pressure and flow configuration
Why choose 114PN
Pressure myography for laboratories that do not need longitudinal force
The 114PN retains the core pressure, cannulation, heating and vessel-tracking workflow of the 114P while omitting the force transducer and associated positioning mechanism.
Static pressure and flow
Study intact vessels under controlled transmural pressure and add the FlowMeter – 162FM where flow measurement is required.
Cannulated vessel preparation
Mount small arteries, veins and other vessels between two cannulas while preserving tubular vessel geometry.
Integrated heating
Maintain chamber temperature without relying on continuous superfusion as the only heating method.
Acid-resistant chamber
Stainless-steel construction supports routine cleaning and helps reduce adhesion of lipids and compounds.
MyoVIEW live tracking
Measure vessel and lumen diameter, wall thickness, pressure and calculated vascular parameters throughout the protocol.
System overview
A simplified 114P-based platform for pressure and diameter endpoints
A small intact artery or vein is mounted onto two cannulas and pressurized to the required transmural pressure. This preserves tubular vessel geometry while allowing diameter, lumen, wall dimensions and pressure-dependent responses to be monitored.
Compared with the 114P, the 114PN removes longitudinal force measurement. It is therefore suited to experiments where vessel diameter, wall structure, pressure response or flow-related function are the relevant endpoints.
Constant pressure
Maintain controlled transmural pressure from 0 to 250 mmHg.
Optional luminal flow
Add FlowMeter – 162FM for flow measurements from 15 to
4,000 µL/min.
Optical base window
Visualize the mounted vessel and support live diameter tracking.
XYZ alignment
Position the cannulated vessel for reliable visualization and
edge detection.
Pressure Interface
Control pressure, chamber heating, calibration and system data.
114PN chamber
Cannula-based chamber without longitudinal force measurement
The 114PN chamber is designed for mounting pressurized vessels between two cannulas. Integrated pressure transducers, XYZ alignment and an optical base window support controlled pressure, visualization and flow-related studies.
Mounting
Glass or compatible stainless-steel cannulas for vessels above
40 µm.
Alignment
X, Y and Z positioning with 0.01 mm micrometer resolution.
Pressure monitoring
In-line inlet and outlet pressure transducers.
Force endpoint
No longitudinal force transducer in the 114PN configuration.
Research applications
Myogenic response, flow-mediated dilation and vascular pharmacology
The 114PN is suited to studies where the biological question depends on the behavior of an intact pressurized vessel and longitudinal force measurement is not required.
01
Myogenic responses
Study pressure-dependent constriction and dilation under controlled transmural pressure.
02
Flow-mediated dilation
Investigate endothelial responses to controlled luminal flow when configured with a FlowMeter.
03
Pharmacological studies
Measure diameter changes following luminal or abluminal exposure to vasoactive compounds.
04
Pressure-diameter relationships
Track vessel and lumen dimensions during controlled pressure steps.
05
Vascular resistance and shear stress
Calculate flow-dependent parameters when the system is paired with the relevant flow workflow.
System components
Chamber, Pressure Interface and MyoVIEW software
The 114PN workflow combines a cannula-based pressure chamber, pressure-control interface and software for live tracking and analysis.
114PN chamber
Cannula-based chamber for pressurized-vessel studies without longitudinal force measurement.
Pressure Interface
Interface with heated reservoir, touch-screen pressure control, calibration procedures, chamber heating and direct data communication.
MyoVIEW software
Software workflow for live vessel detection, dimension tracking, pressure acquisition and pressure-myograph calculations.
MyoVIEW workflow
Live diameter tracking and pressure-myograph analysis
MyoVIEW collects vessel-wall thickness, vessel and lumen diameter, intravascular pressure and calculated parameters throughout pressure, flow and pharmacological protocols.
Live detection
Track vessel and lumen diameter continuously during the
experiment.
Multiple scanlines
Use several edge-detection zones for reliable vessel
measurement.
Protocol markers
Mark compounds, pressure steps, flow changes and other
interventions.
Calculated outputs
Review diameter, wall thickness, area, flow, vascular
resistance and shear stress.
Technical data
Key 114PN specifications
Core chamber, pressure, temperature, reservoir and communication specifications for evaluating the 114PN.
The 114PN is optimized for pressure, vessel-dimension and optional
flow studies without longitudinal force measurement.
Chamber
- Number of chambers
- 1
- Minimum chamber volume
- 3.1 mL
- Chamber material
- Acid-resistant stainless steel
- Vessel size
- >40 µm
- Vessel alignment
- X, Y and Z
- Micrometer resolution
- 0.01 mm
- Mounting type
- Cannulas
Temperature
- Temperature range
- 15.0–50.0 °C
- Resolution
- 0.1 °C
- Stability
- ±0.2 °C
- Integrated heating
- Yes
Pressure
- Output reading
- mmHg
- Pressure range
- 0–250 mmHg
- Pressure stability
- ±0.5 mmHg
- Pressure resolution
- 0.1 mmHg
Reservoir
- Heating
- Yes
- Capacity
- 250 mL
- Pressure circuit
- Closed
- Air inlet
- 1 bar maximum
Output
- Data communication
- USB 2.0
- Analogue output channels
- 4
- Analogue output range
- ±2.5 V
System distinction
- Longitudinal force transducer
- Not included
- Primary endpoints
- Pressure, diameter, wall and flow
Configuration guidance
Plan the complete 114PN pressure-myography workflow
The appropriate configuration depends on vessel dimensions, microscope and camera compatibility, cannula format, pressure circuit, optional flow measurement, software requirements and laboratory training needs.
Need-to-have equipment
Typical workflows include MyoVIEW, computer package, inverted microscope, camera, stage micrometer, vacuum package, heating bath or circulator, dissection microscope, light source and dissection tools.
Flow and mounting options
Confirm cannula dimensions, pressure tubing, peristaltic pump requirements and FlowMeter – 162FM where flow measurement is part of the protocol.
Training and service
Installation, cannulation training, pressure calibration, leak testing, spare pressure transducers and maintenance coverage help support consistent operation.
Frequently asked questions
Common questions about the 114PN
What is the Pressure Myograph System 114PN used for?
The 114PN is designed to study small arteries and veins under near-physiological pressure conditions without longitudinal force measurement. It allows researchers to measure vessel diameter, wall thickness, myogenic responses, endothelial function, and flow-dependent vascular behavior in a more economical configuration.
What types of blood vessels can be studied with the 114PN?
The 114PN is designed for small arteries, veins, and other cannulated vessels larger than 40 µm. It preserves the vessel's natural tubular structure, making it ideal for pressure- and flow-based vascular physiology studies.
What measurements can the Pressure Myograph System 114PN perform?
The system measures vessel diameter, lumen diameter, wall thickness, intravascular pressure, pressure-diameter relationships, myogenic tone, and flow-mediated responses. When used with MyoVIEW, researchers can also calculate vascular resistance, shear stress, and other derived vascular parameters.
Can the 114PN be used to study myogenic tone and flow-mediated dilation?
Yes. The 114PN is ideal for myogenic tone, flow-mediated dilation, endothelial function, vascular reactivity, and pharmacological studies where vessel diameter and pressure are the primary experimental endpoints. Optional FlowMeter integration supports controlled luminal flow experiments.
Why choose the 114PN over the 114P?
The 114PN offers the same pressure myography workflow as the 114P but does not include longitudinal force measurement. It's the ideal choice for laboratories focused on vessel diameter, pressure, wall structure, and flow studies that don't require axial force data, making it a more cost-effective solution.
What research applications is the 114PN best suited for?
The 114PN is widely used in vascular biology, hypertension, endothelial dysfunction, flow-mediated dilation, vascular remodeling, pharmacology, and microvascular research where pressure- and diameter-based measurements are the primary focus.
Do I need longitudinal force measurement for pressure myography?
Not always. If your research focuses on vessel diameter, pressure, flow, endothelial function, or myogenic responses, the 114PN provides all the essential pressure myography capabilities without longitudinal force measurement. If your protocols require measuring axial vessel tension, the 114P is the better choice.
Is the Pressure Myograph System 114PN the right system for my research?
The 114PN is an excellent choice if your research focuses on small-vessel pressure myography, flow-mediated studies, vascular reactivity, and vessel diameter measurements without the need for longitudinal force data. If your experiments require axial force measurements, consider the 114P. If you need pulsatile pressure simulation, the 112PP is the better option.
Resources
114PN documentation and supporting resources
Use the official DMT documents and related pages when evaluating, configuring or operating the 114PN.
114PN data sheet
Product overview, applications and technical specifications.
→114PN user manual
Detailed system setup, pressure circuit, interface and calibration documentation.
→114PN user guide
Practical reference for mounting and routine system operation.
→MyoVIEW user manual
Software documentation for pressure-myograph tracking and analysis.
→MyoVIEW software
Live vessel tracking and analysis for DMT pressure myographs.
→FlowMeter – 162FM
Optional flow measurement for compatible pressure-myograph protocols.
→Pressure comparison chart
Compare the 114PN with other DMT pressure-myograph systems.
→Training and installation
Plan system setup, cannulation, calibration and user onboarding.
→
Configuration guidance
Planning pressure or flow studies without longitudinal force?
Share the vessel type and diameter, pressure range, flow needs, microscope and camera setup, cannula preference and intended MyoVIEW analysis. DMT can help confirm whether the 114PN is the appropriate pressure-myograph configuration.