Blind-sac chamber design
Mount the vessel between a glass cannula and a steel pin rather than between two cannulas.
Pressure Myograph System – 114PB
Blind-sac pressure myography for small isolated vessels
The DMT 114PB is a specialized pressure myograph for studying isolated small vessels in a blind-sac configuration under controlled, near-physiological pressure. The preparation is mounted between a glass cannula and a mounting steel pin, while vessel diameter, lumen, wall structure and pressure-dependent responses are recorded with MyoVIEW.
Vessels >40 µm
Small isolated vessels under controlled pressure.
Blind-sac mounting
Glass cannula combined with a mounting steel pin.
MyoVIEW ready
Live vessel tracking and pressure-myograph analysis.
Blind-sac pressure myograph
Why choose 114PB
Purpose-built for constant-pressure blind-sac experiments
The 114PB is intended for studies where the vessel preparation or protocol requires a closed-end mounting configuration and where pressure-dependent diameter and wall responses are the principal experimental endpoints.
Controlled transmural pressure
Study intact vessels under stable pressure while preserving their tubular geometry.
Relevant for myogenic tone
Evaluate physiological and pharmacological responses where pressure-dependent vascular tone is central.
Integrated chamber heating
Maintain stable temperature without relying on continuous superfusion as the sole heating method.
Optical vessel access
Observe vessel diameter and wall behavior through the chamber base window.
MyoVIEW analysis
Record vessel and lumen diameter, wall thickness, pressure and calculated vascular parameters.
System overview
A closed-end mounting pathway for pressurized vessel studies
In the 114PB, one end of the isolated vessel is mounted on a glass cannula connected to the pressure pathway. The opposite end is secured to a mounting steel pin, creating the blind-sac preparation.
The chamber supports pressure control, vessel visualization and XYZ alignment. Its cover includes ports for optional superfusion, rapid filling and draining, oxygenation and cumulative compound addition.
Mounting principle
Glass cannula on the pressure side and steel pin on the blind
side.
Constant pressure
Maintain controlled intravascular pressure between 0 and
250 mmHg.
XYZ alignment
Position the mounted vessel for visualization and reliable edge
detection.
Chamber access
Use the cover ports for superfusion, oxygenation, draining,
filling and drug addition.
Pressure Interface
Manage pressure regulation, calibration, heating and direct data
communication.
System components
Chamber, blind-sac mount and pressure interface
The complete workflow combines the specialized chamber, mounting components and interface required for controlled blind-sac pressure myography.
114PB chamber with cover
Chamber cover with access for optional superfusion, rapid filling and draining, oxygenation and cumulative compound addition.
Blind-sac mounting area
Vessel mounting arrangement with glass cannula, mounting steel pin, pressure connection and adjustable vessel positioning.
Pressure Interface
Interface with touch-screen control, heated reservoir, pressure regulation, calibration, chamber heating and data outputs.
Research applications
Pressure-dependent function in blind-sac vessel preparations
The 114PB is relevant where the biological question requires an intact pressurized vessel but the specimen or protocol is better suited to a single-cannula, closed-end configuration.
01
Myogenic tone
Study pressure-dependent constriction and dilation in an intact tubular vessel preparation.
02
Pharmacological responses
Measure vessel-diameter responses to vasoactive compounds and other experimental interventions.
03
Pressure-diameter studies
Record changes in vessel and lumen diameter during controlled pressure steps.
04
Vessel wall analysis
Collect wall-thickness and lumen measurements during pressure-dependent protocols.
05
Fluorescence workflows
Combine pressure myography with compatible imaging of intracellular calcium and related vascular signals.
MyoVIEW workflow
Live vessel tracking and pressure-myograph analysis
MyoVIEW provides continuous visualization and recording of the mounted vessel throughout constant-pressure, pressure-step and pharmacological protocols.
Vessel diameter
Track changes in external vessel diameter during the
experiment.
Lumen diameter
Record changes in the vessel lumen under pressure and
pharmacological intervention.
Wall thickness
Measure vessel-wall dimensions and structural responses.
Pressure data
Record intravascular pressure together with vessel-dimension
measurements.
Calculated parameters
Review derived vascular parameters relevant to the selected
protocol.
Technical data
Key 114PB specifications
Core chamber, mounting, temperature, pressure, reservoir and communication specifications for evaluating the 114PB.
Confirm the vessel type, preparation dimensions, mounting-pin
size, cannula format, microscope, camera, pressure pathway and
MyoVIEW configuration before ordering.
Chamber
- Number of chambers
- 1
- Minimum chamber volume
- 3.1 mL
- Chamber material
- Acid-resistant stainless steel
- Vessel size
- >40 µm
- Vessel alignment
- X, Y and Z
- Micrometer resolution
- 0.01 mm
- Mounting type
- Glass cannula and blind-sac steel pin
Temperature
- Temperature range
- 15.0–50.0 °C
- Resolution
- 0.1 °C
- Stability
- ±0.2 °C
- Integrated heating
- Yes
Pressure
- Output reading
- mmHg
- Pressure range
- 0–250 mmHg
- Pressure stability
- ±0.5 mmHg
- Pressure resolution
- 0.1 mmHg
Reservoir
- Heating
- Yes
- Capacity
- 250 mL
- Pressure circuit
- Closed
- Air inlet
- 1 bar maximum
Output
- Data communication
- USB 2.0
- Analogue output channels
- 4
- Analogue output range
- ±2.5 V
Configuration guidance
Plan the complete blind-sac pressure-myography setup
The correct configuration depends on vessel type, mounting-pin diameter, cannula format, microscope and camera pathway, pressure circuit, software requirements and user experience.
Need-to-have equipment
Typical workflows include MyoVIEW, a suitable computer, inverted microscope, camera, stage micrometer, vacuum package, heating bath or circulator, dissection microscope, light source and dissection tools.
Blind-sac mounting items
Confirm mounting steel-pin dimensions, glass or stainless-steel cannulas, suture, installation kit, gas supply manifold, tubing and pressure transducer requirements.
Training and service
Installation, vessel-mounting training, pressure calibration, leak testing, spare pressure transducers and maintenance coverage help support consistent daily operation.
Frequently asked questions
Common questions about the 114PB
What is the Pressure Myograph System 114PB used for?
The 114PB is designed for blind-sac pressure myography, allowing researchers to study small blood vessels under controlled pressure using a single cannula and a closed-end mounting configuration. It is ideal for experiments where traditional two-cannula mounting is not practical.
What types of blood vessels can be studied with the 114PB?
The 114PB is designed for small arteries, veins, and other isolated vessels larger than 40 µm that require a blind-sac mounting configuration. It preserves the vessel's natural tubular structure while allowing precise pressure control and diameter measurements.
What measurements can the Pressure Myograph System 114PB perform?
The system measures vessel diameter, lumen diameter, wall thickness, intravascular pressure, myogenic responses, and pressure-dependent changes in vascular function. MyoVIEW software provides live vessel tracking and calculated vascular parameters throughout the experiment.
When should I use a blind-sac pressure myograph?
A blind-sac pressure myograph is the preferred choice when your vessel preparation or experimental protocol requires only one cannulated end while maintaining controlled intravascular pressure. This configuration is useful for specialized pressure-dependent studies where standard two-cannula mounting is not suitable.
Can the 114PB be used to study myogenic tone and vascular reactivity?
Yes. The 114PB is well suited for myogenic tone, vascular reactivity, pressure-diameter relationships, endothelial function, and pharmacological studies under controlled pressure conditions. It can also be combined with compatible fluorescence imaging workflows.
What research applications is the 114PB best suited for?
The 114PB is commonly used in vascular physiology, hypertension, endothelial function, vascular remodeling, pharmacology, pressure-dependent vessel mechanics, and fluorescence imaging where a blind-sac preparation is required.
What's the difference between the 114PB and the 114P Pressure Myograph?
The 114P mounts vessels between two cannulas, making it the standard choice for most pressure myography studies. The 114PB uses a single cannula and a blind-sac mounting pin, making it the preferred system for specialized vessel preparations or protocols that require a closed-end configuration.
Is the Pressure Myograph System 114PB the right system for my research?
The 114PB is the right choice if your experiments require blind-sac mounting, controlled intravascular pressure, and pressure-dependent vessel measurements. For conventional two-cannula pressure myography, the 114P is typically the better option, while the 112PP is recommended when pulsatile pressure simulation is required.
Resources
114PB documentation and supporting resources
Use the official DMT documentation and related support pages when planning, installing or operating the 114PB.
114PB product data sheet
Product overview, applications and core technical specifications.
→114PB user manual
Detailed system setup, pressure-interface, calibration and connection guidance.
→114PB user guide
Practical workflow reference for routine system operation.
→MyoVIEW software
Live vessel tracking and analysis for DMT pressure myographs.
→Pressure myograph systems
Review the complete DMT pressure-myograph product family.
→Pressure comparison chart
Compare the 114PB with other pressure-myograph configurations.
→Pressure how-to videos
Access calibration, cannula, cover-glass and leak-check guidance.
→Training and installation
Plan setup, vessel mounting, calibration and user onboarding.
→
Configuration guidance
Planning a blind-sac pressure-myography study?
Share the vessel type and size, available preparation length, pressure range, mounting-pin requirement, cannula preference, microscope and camera setup and intended MyoVIEW analysis. DMT can help define the appropriate 114PB configuration.