Near-physiological conditions
Study intact tubular vessels under controlled transmural pressure rather than as ring-mounted preparations.
Pressure Myograph System – 114P
Pressure myography for small isolated vessels under near-physiological conditions
The DMT 114P is a pressure myograph for studying the structure and function of isolated small arteries, veins and other vessel segments under controlled transmural pressure. The system supports myogenic-response studies, flow-mediated dilation, pharmacological testing, longitudinal force measurement and live vessel tracking with MyoVIEW.
Vessels >40 µm
Cannulated small-vessel preparations.
±200 mN force
Longitudinal force measurement alongside pressure.
MyoVIEW ready
Live diameter, lumen and wall analysis.
Standard small-vessel platform
Why choose 114P
A versatile platform for pressure, diameter, force and flow-related studies
The 114P is designed for laboratories that need a reliable, adaptable pressure-myograph system for small-vessel physiology, vascular pharmacology and functional analysis.
Precise cannula alignment
XYZ adjustment and ergonomic chamber geometry support controlled and reproducible vessel mounting.
Longitudinal force measurement
Measure axial vessel force independently of pressure and diameter control.
Integrated heating
Maintain chamber temperature without relying on continuous superfusion for heat control.
Low-volume chamber
A minimum chamber volume of 3.1 mL helps reduce buffer and compound consumption.
Imaging compatible
Combine live diameter tracking with fluorescence measurements such as intracellular calcium or pH.
System overview
Cannulated vessel studies with pressure, force and diameter endpoints
A small intact segment of an artery or vein is mounted onto two cannulas and pressurized to the required transmural pressure. This preserves tubular vessel geometry while allowing diameter, pressure, wall dimensions and longitudinal force to be monitored.
The chamber cover includes ports for superfusion, rapid filling and draining, cumulative drug addition and oxygenation. The optical base window supports continuous visualization and MyoVIEW-based vessel tracking.
Constant pressure
Maintain controlled transmural pressure from 0 to 250 mmHg.
Optional luminal flow
Add FlowMeter – 162FM for flow measurement from 15 to
4,000 µL/min.
Longitudinal force
Record axial force independently from pressure and diameter.
Optical base window
Visualize the mounted vessel and support live dimension tracking.
Closed pressure circuit
Use the heated 250 mL reservoir and Pressure Interface for
controlled operation.
114P chamber
Compact chamber design for precise small-vessel mounting
The acid-resistant stainless-steel chamber supports cannulated vessel mounting, integrated pressure measurement, longitudinal force recording, XYZ alignment and visualization through the optical base window.
Mounting
Glass or stainless-steel cannulas for vessels above 40 µm.
Alignment
X, Y and Z adjustment with 0.01 mm micrometer resolution.
Force measurement
Longitudinal force range of ±200 mN with 0.01 mN resolution.
Chamber handling
Ports support filling, draining, superfusion, oxygenation and
cumulative compound addition.
Research applications
Small-vessel physiology, pharmacology and vascular mechanics
The 114P is used when the research question depends on the behavior of an intact pressurized vessel rather than a ring-mounted preparation.
01
Myogenic responses
Study pressure-dependent constriction and dilation under controlled transmural pressure.
02
Flow-mediated dilation
Investigate endothelium-dependent responses to controlled luminal flow.
03
Pharmacological testing
Apply vasoactive compounds to the superfusate or luminal solution and measure changes in vessel diameter.
04
Vascular mechanics
Combine diameter, pressure and longitudinal force data during vessel-stretch or pressure protocols.
05
Fluorescence studies
Combine pressure myography with imaging of intracellular calcium, pH or related vascular signals.
MyoVIEW workflow
Live vessel tracking and pressure-myograph analysis
MyoVIEW records vessel-wall thickness, vessel and lumen diameter, intravascular pressure and calculated vascular parameters during pressure, flow and pharmacological protocols.
Live detection
Track vessel and lumen diameter continuously during the
experiment.
Multiple scanlines
Use several edge-detection zones to improve measurement
reliability.
Protocol markers
Mark pressure steps, compounds, flow changes and other
experimental events.
Calculated outputs
Review wall thickness, cross-sectional area, vascular
resistance, shear stress and related parameters.
Technical data
Key 114P specifications
Core chamber, pressure, force, temperature and communication specifications for evaluating the 114P.
Confirm vessel dimensions, cannula type, microscope and camera
setup, MyoVIEW workflow, optional flow measurement and training
requirements before ordering.
Chamber
- Number of chambers
- 1
- Minimum chamber volume
- 3.1 mL
- Chamber material
- Acid-resistant stainless steel
- Vessel size
- >40 µm
- Vessel length
- 1–10 mm
- Vessel alignment
- X, Y and Z
- Mounting type
- Cannulas
Temperature
- Temperature range
- 15.0–50.0 °C
- Resolution
- 0.1 °C
- Stability
- ±0.2 °C
- Integrated heating
- Yes
Longitudinal force
- Output reading
- mN
- Force range
- ±200 mN
- Force resolution
- 0.01 mN
- Force calibration
- Yes
Pressure
- Output reading
- mmHg
- Pressure range
- 0–250 mmHg
- Pressure stability
- ±0.5 mmHg
- Pressure resolution
- 0.1 mmHg
Reservoir
- Heating
- Yes
- Capacity
- 250 mL
- Pressure circuit
- Closed
- Air inlet
- 1 bar maximum
Output
- Data communication
- USB 2.0
- Analogue output channels
- 4
- Analogue output range
- ±2.5 V
Configuration guidance
Plan the complete 114P pressure-myography setup
The appropriate configuration depends on vessel dimensions, microscope and camera compatibility, cannula format, pressure circuit, optional flow measurement, imaging requirements and laboratory training needs.
Need-to-have equipment
Typical workflows include MyoVIEW, computer package, inverted microscope, monochrome camera, stage micrometer, vacuum package, heating bath or circulator and dissection equipment.
Mounting and flow options
Confirm glass or stainless-steel cannula sizes, pressure tubing, peristaltic pump requirements and FlowMeter – 162FM where flow measurement is required.
Specialized options and support
Rapid-freezing studies can use the 115FP chamber. Installation, cannulation training, spare pressure transducers and service coverage support reliable operation.
Frequently asked questions
Common questions about the 114P
What is the Pressure Myograph System 114P used for?
The 114P is used to study small arteries and veins under near-physiological pressure conditions. It enables researchers to measure vascular function, vessel diameter, myogenic responses, endothelial function, and pharmacological effects while preserving the vessel's natural tubular structure.
What types of blood vessels can be studied with the 114P?
The 114P is designed for small arteries, veins, and other cannulated vessels larger than 40 µm in diameter. It is ideal for resistance vessel studies where maintaining physiological pressure is essential for accurate measurements.
What measurements can the Pressure Myograph System 114P perform?
The 114P measures vessel diameter, wall thickness, myogenic tone, longitudinal force, pressure-diameter relationships, and vascular responses to pharmacological and physiological stimuli. When paired with MyoVIEW, researchers can also calculate vascular resistance, shear stress, and other derived parameters.
Can the 114P be used to study myogenic tone and flow-mediated dilation?
Yes. The 114P is specifically designed for myogenic tone, flow-mediated dilation, endothelial function, vascular reactivity, and pressure-dependent physiology. Optional flow measurement allows researchers to investigate endothelial responses under controlled luminal flow conditions.
Why choose the 114P over a Wire Myograph?
A Pressure Myograph studies intact, pressurized blood vessels, allowing researchers to measure changes in vessel diameter and function under near-physiological conditions. A Wire Myograph measures isometric force in ring-mounted vessels and is better suited for vascular contractility studies. The 114P is the preferred choice when vessel pressure and diameter are key experimental endpoints.
What research applications is the 114P best suited for?
The 114P is widely used in vascular biology, hypertension, diabetes, endothelial dysfunction, vascular remodeling, cardiovascular disease, pharmacology, and microvascular research where small-vessel function is a primary focus.
What's the difference between the 114P and the 112PP Pressure Myograph?
Both systems study small pressurized blood vessels, but the 114P is designed for standard pressure myography with optional flow and longitudinal force measurement. The 112PP adds programmable pulsatile pressure simulation, making it the better choice for researchers who need to replicate pulsatile blood flow or investigate dynamic vascular mechanics.
Is the Pressure Myograph System 114P the right system for my research?
The 114P is the ideal choice if you're studying small-vessel physiology, vascular reactivity, endothelial function, myogenic tone, or pharmacological responses under controlled pressure conditions. If your research requires pulsatile pressure, consider the 112PP. If you don't require longitudinal force measurements, the 114PN offers a streamlined alternative.
Resources
114P documentation and supporting resources
Use the official DMT documents and related pages when evaluating, configuring or operating the 114P.
114P product data sheet
Product overview, applications, specifications and configuration guidance.
→112PP/114P user manual
Detailed setup, pressure circuit, interface and calibration documentation.
→112PP/114P user guide
Practical reference for routine pressure-myograph operation.
→MyoVIEW user manual
Software documentation for vessel tracking and analysis.
→MyoVIEW software
Live vessel tracking and analysis for pressure-myograph workflows.
→FlowMeter – 162FM
Optional flow measurement for pressure-myograph protocols.
→Pressure comparison chart
Compare vessel size, flow, force, pulsation and imaging capability.
→Training and installation
Plan system setup, cannulation, calibration and user onboarding.
→
Configuration guidance
Planning a small-vessel pressure-myography study?
Share the vessel type and diameter, pressure range, longitudinal force requirements, flow needs, microscope and camera setup, cannula preference and analysis workflow. DMT can help define the appropriate 114P configuration.