WIRE MYOGRAPH SYSTEM - GENERAL
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FEATURES & BENEFITS
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The Wire Myograph allows examination of small vessels (internal diameter 60 μm - 10 mm) in terms of morphology and responsiveness to hormones and other agonists.
The small vessels are mounted as ring preparations by threading them onto two stainless steel or tungsten wires and securing the wires to two supports.
One support is then attached to a micrometer, allowing control of vessel circumference. The other support is attached to a force transducer for measurement of tension development. The whole preparation is kept in a chamber with physiological salt solution at 37°C, bubbled with oxygen. Vessels maintained in Wire Myographs are viable for several hours.
The small vessels are mounted as ring preparations by threading them onto two stainless steel or tungsten wires and securing the wires to two supports.
One support is then attached to a micrometer, allowing control of vessel circumference. The other support is attached to a force transducer for measurement of tension development. The whole preparation is kept in a chamber with physiological salt solution at 37°C, bubbled with oxygen. Vessels maintained in Wire Myographs are viable for several hours.
Following mounting and equilibration, the passive length-tension relationships of the vessels are determined; a normalization procedure. During the actual experiments, the circumference of the vessels are kept constant and vessels are examined under isometric conditions. Compounds are added directly to the chamber and vessel tension is monitored. Furthermore, it is possible to compare vessels from patients or test groups with those of control, not only in terms of vessel reactivity to various compounds, but also in terms of morphology.
The following lists are a few of the established areas of investigation for Wire Myograph systems. Many more investigation possibilities for vascular and other smooth muscle may be added through the imagination of researchers such as yourself.
The following lists are a few of the established areas of investigation for Wire Myograph systems. Many more investigation possibilities for vascular and other smooth muscle may be added through the imagination of researchers such as yourself.
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BASIC TISSUE PROPERTIES
TISSUES USED
VASOACTIVE MECHANISMS
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PHARMACOLOGY & PHARMACOTHERAPY
FURTHER POSSIBILITIES
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ALL WIRE MYOGRAPH SYSTEMS |
Single Wire Myograph System - 320A
Ideal for studying a single vessel with a diameter of 60 μm - 3 mm. The vessel is mounted as a ring preparation by threading it over two parallel stainless steel wires and securing the wires to two supports or "jaws". Dual Wire Myograph System - 420A & 520A Simultaneous testing of two vessels with diameters of 60 μm - 3 mm, independently. Multi Wire Myograph System - 620M This 4-channel multi myograph system is a highly sophisticated yet robust research instrument. It is an easy-to-use system for in vitro studies of small and large blood vessels, trachea or gut mounted as larger ring preparations (up to 10 mm). Multi Wire Myograph System - 630MA The newly developed 4-channel system adds the ease of automating the normalization procedures so that calculations and preload tension is easily set. Following mounting and equilibration, passive length-tension relationships are determined by a standardized procedure. Confocal Wire Myograph System 360CW Specially designed to provide very close optical access to the mounted artery or tissue segment, thereby allowing high resolution images of fluorescent dyes or markers by laser scanning confocal microscopy (LSCM). |
RESEARCH VIDEO
"Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography" (520A)
(subscription TO JoVE is required)
Lakeesha E. Bridges, Cicely L. Williams, Mildred A. Pointer, Emmanuel M. Awumey
"Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography" (520A)
(subscription TO JoVE is required)
Lakeesha E. Bridges, Cicely L. Williams, Mildred A. Pointer, Emmanuel M. Awumey
