Standard Reactivity Experimental Protocol
General vascular reactivity guidance covering equilibration, wake-up procedures, functional testing and concentration-response experiments using a DMT wire myograph.
DMT Academy · Scientific Resources
Protocols, articles and scientific guidance for ex vivo physiology
Access practical experimental protocols, application articles and selected research resources for DMT wire myographs, pressure myographs, organ bath systems and ex vivo muscle physiology workflows.
Experimental protocols
Starting points for vascular, muscle and organ bath studies.
Application guidance
Connect system choice with preparation and endpoint.
Scientific context
Articles and references supporting experimental planning.
Use this page for planning
Match the resource to the experiment
A useful protocol must fit the system family, preparation, species, tissue size, measurement endpoint and laboratory workflow.
System family
Wire, pressure, organ bath or muscle-strip platform.
Preparation
Vessel ring, cannulated vessel, tissue strip or muscle.
Endpoint
Force, diameter, pressure, flow or electrophysiology.
Research question
Reactivity, contraction, structure, screening or mechanics.
Protocols are general starting points and may require adaptation
for the selected species, tissue, compound and experimental
objective.
Browse by resource type
Choose the scientific content you need
Begin with a protocol for experimental structure, use application articles for system and workflow context, and continue to manuals, videos or training for practical implementation.
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Featured protocols
Practical starting points for common wire, pressure and organ bath experimental workflows.
View featured protocols PDFProtocol library
Download the complete collection of currently published DMT protocol and application PDFs.
Browse PDF library ARTSelected articles
Read DMT application articles, researcher profiles and scientific method discussions.
Read selected articles SYSBrowse by workflow
Connect resources with wire myography, pressure myography, organ bath and muscle-strip systems.
Find a workflowFeatured protocols
Practical starting points for common experimental workflows
These resources provide general experimental structure and background for vascular reactivity and smooth-muscle contractility studies.
Standard Reactivity Experimental Protocol
General protocol guidance for pressurized vessel studies, including equilibration, functional testing, pressure curves, concentration responses and flow-mediated dilation.
Investigation of Smooth Muscle Contraction by Organ Bath
Application-oriented guidance for organ bath experiments investigating smooth-muscle contraction and pharmacological responses.
Protocol library
Download the current scientific resource PDFs
The library includes vascular reactivity protocols, organ bath application material and ex vivo muscle electrophysiology guidance.
Standard Reactivity Experimental Protocol – Wire Myography
Vascular reactivity guidance for mounted vessel rings and the DMT 620M workflow.
→Standard Reactivity Experimental Protocol – Pressure Myography
Pressurized vessel experiments, pressure curves and flow-mediated dilation.
→Investigation of Smooth Muscle Contraction by Organ Bath
Organ bath workflow and smooth-muscle contraction guidance by Prof. Martin Hennenberg.
→Identifying New Compounds for Inhibition of Smooth Muscle Contraction
Application guide for compound evaluation using organ bath experiments.
→Electrophysiology Studies of Muscle Ex Vivo Protocols
Ex vivo muscle physiology and electrophysiology guidance from Dr. Thomas Kragh.
→Selected articles
Research perspectives and application discussions
Read selected DMT articles covering vascular research, automation and the choice between wire and pressure myography.
Researcher profile
Meet Dr. Michael Hill – A Pioneer in Vascular Research
A profile exploring vascular research, scientific development and the work of an established investigator in the field.
Open article AutomationThe Ultimate Wire Myograph & Organ Bath: Robotics & Automation
An application article discussing automation, laboratory throughput and advanced wire-myograph and organ bath workflows.
Open article System selectionInvestigating Vascular Disease: Wire or Pressure Myography?
A practical discussion of the different experimental questions addressed by wire and pressure myography in animal models.
Open articleBrowse by workflow
Connect resources with the relevant DMT system family
Use the workflow cards to move from scientific planning to product information, operating guidance and support.
Wire myography
For vascular rings and other mounted preparations where isometric force is the primary endpoint.
- Standard wire-myography reactivity protocol
- Equilibration and wake-up procedures
- Concentration-response experiments
- Wire versus pressure myography article
Pressure myography
For intact cannulated vessels where pressure, diameter, structure, flow or myogenic responses are measured.
- Standard pressure-myography reactivity protocol
- Pressure curves and myogenic response
- Flow-mediated dilation considerations
- Wire versus pressure myography article
Organ bath studies
For isolated tissue and smooth-muscle contraction experiments, pharmacology and compound screening.
- Smooth-muscle contraction guidance
- Compound inhibition application guide
- Contractility and pharmacology workflows
- Wire-myograph versus organ-bath context
Ex vivo muscle physiology
For muscle-strip, electrophysiology and tissue-mechanics workflows using isolated muscle preparations.
- Ex vivo electrophysiology protocols
- Muscle-strip system context
- Stimulation and controller software
- Training and experimental setup support
Using scientific resources
Adapt protocols to the preparation and research question
Published DMT protocols provide general experimental structure and practical starting points. They are not universal methods for all tissues, species, compounds or disease models.
Laboratories should define their own inclusion criteria, controls, concentrations, timing and analysis procedures based on the biological question and established scientific practice.
Use protocol PDFs together with the current system manual, software
documentation, laboratory SOPs and relevant peer-reviewed
literature.
System compatibility
Confirm that the protocol matches the selected myograph, chamber,
accessories and software workflow.
Tissue and species
Adapt mounting, resting conditions, agonists, concentrations and
timing to the preparation.
Experimental controls
Define functional tests, inclusion criteria, negative controls
and positive controls before data collection.
Data analysis
Establish the analysis method, normalization approach and
reporting format before beginning the study.
Training needs
Combine written protocols with practical training when users are
new to the preparation or system.
Related Academy resources
Continue from scientific planning to practical system use
Pair protocols and articles with videos, manuals, software and hands-on training.
Scientific resource guidance
Need help identifying the most relevant protocol or article?
Tell DMT which system, tissue preparation, species, measurement endpoint, software workflow and research application you are working with. We can help direct you to the most relevant available material and support route.