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Low volume to minimize reagent use
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Precise 3-axis positioning of cannulae
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Live tracking and data streaming
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Reliable, flexible & proven technology
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The Imaging Pressure Myograph System - 120CP is a system designed specifically for use with laser scanning imaging microscopes (LSCM) and other high magnification microscopes for imaging studies where intracellular processes within the smooth muscle cells or endothelial cells of the intact, pressurized vessel are needed. This system is ideal for studying intracellular ion concentrations such as calcium or tracking the trafficking of fluorescent probes, tags or proteins in small vessels (internal diameter >40 μm). However the chamber can still be used for standard pressure myography.
The conical open bath design allows easy access for high magnification/high numerical aperture lenses used in inverted microscopes. It also allows access for direct immersion lenses used in upright imaging microscopy. In addition, the top part of the myograph can be easily lifted, facilitating maintenance and mounting. Specially designed and angled mounting supports in combination with a z-axis manipulator facilitate precise vertical positioning of an isolated blood vessel directly above or on top of the chamber window, permitting the use of objectives with working distances as low as 100. The intravascular pressure and the pressure gradient along the vessel can be precisely controlled. A built-in heating system maintains bath temperature above ambient temperature. The customized chamber cover includes ports for super fusion, for rapid draining and filling, and for gas bubbling. To facilitate cleaning, the chamber is made of acid-resistant stainless steel. When using the myograph on an imaging microscope, data acquisition of inlet and outlet pressure, force and temperature is possible by using either the analog outputs or the serial interface. |
Related Products:
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Myograph Chamber # Study the structure and function of small vessels >40 μm under near-physiological conditions # Specifically designed for use with laser scanning microscopes and imaging systems # Ideal for studying intracellular ion concentrations and isobaric constriction # Designed to accommodate objective working distances as low as 100 μm # In-line pressure transducers # XYZ alingment of pressurized vessel # Acid resistant stainless steel chamber |
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Interface Unit # Integrated buffer reservoir (heated) # Direct data streaming # Integrated heat control # Integrated force and pressure calibration procedure # Intuitive touch-screen menus # Compact design / small footprint |
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Chamber:
Chamber volume (min): Chamber(s): Chamber material: Vessel size: Vessel alignment: Micrometer resolution: Mounting type: Temperature:
Range: Resolution: Stability: Heating: Transducer Force:
Output reading: Range: Resolution: Force Calibration: Transducer Pressure:
Output reading: Range: Pressure stability: Resolution: Force calibration: RESERVOIR:
Heated: Capacity: Pressure circuit: Air inlet: Output:
Data communication: Analogue output channels: Analogue output range: |
6.5 ml 1 Acid resistant stainless steel >40 µm X, Y, Z 0.01 mm Cannulas
15.0 to 50.0 °C 0.1 °C ±0.2 °C Yes mN ±200 mN 0.01 mN Yes mmHg 0 - 250 mmHg ±0.5 mmHg 0.1 mmHg Yes Yes 250 ml Closed 1 bar (max) USB 2.0 4 ±2.5 V |
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Need-to-have:
Computer Package (Desktop) (#101022) Vacuum Package - 110V (#101018), 220V (#101052) Open Heating Bath Circulator- Water bath 110V (#400738), 220V (#400740) Dissection Stereo Microscope – Zeiss Stemi 305 (#400484) Light Source CL6000 LED- Swan-neck (#400794) Dissection Tool Kit (#101017) |
Need-to-have:
FlowMeter - 162FM (#300144) Installation Kit (#300639) Gas Supply Manifold- 4-channel (#300295) Gas Supply Manifold - 10-channel (#300345) Peristaltic pump – 2- Channel (#400422) Pressure Transducer (#300055) Glass cutter (#400675) Service & Maintenance Agreement (5yr) (#101054) Extended warranty +3 years (#101034) |
