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The Single Wire Myograph System - 310A is ideal for studying a single vessel with a diameter of 60 μm - 3 mm. The vessel is mounted as a ring preparation by threading it over two parallel stainless steel wires and securing the wires to two supports or "jaws". One support is attached to a precision micrometer, allowing manual control of vessel circumference and stretch. The other support is attached to a force transducer for measurements of force/tension development.
»for studying a single vessel with a
diameter of 60 μm - 3 mm«
The preparation is mounted in a heated 10 ml acid-resistant, stainless steel chamber, which can be covered with a lid featuring ports for rapid suction/draining, refilling and bubbling of oxygen. The base of the chamber contains a window allowing morphological observation or fluorescence measurements on an inverted microscope. Typically, the preparation is kept in the heated vessel chamber in a physiological salt solution at 37°C, bubbled with oxygen where the vessels remain viable for at least 12 hours.
»base window allowing morphological
or fluorescence measurements«
Following mounting and equilibration, the passive length-tension relationship of the vessel is determined. During the actual experiment, the circumference of the vessel is kept constant. Compounds can be added directly to the chamber, and the vessel’s contractility and reactivity are measured under isometric conditions.
As an option, an electronic valve can be added to the system for easy control and emptying of the chamber.
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ACCESSORIES
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Stainless steel jaws
The stainless steel jaws are the standard outfit of the myograph as they allow the mounting of small vessels. |
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Chamber cover
Cover with suction/draining, refilling and oxygen supply ports. |
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RESEARCH PAPERS
Climent B, Zsiros E, Stankevicius E, de la Villa P, Panyi G, Simonsen U, GarcÃa-Sacristán A, Rivera L
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Syyong HT, Chung AW, Van Breemen C
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