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Monday |
Tuesday |
Wednesday |
Thursday |
Friday |
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Morning programme |
Welcome:
Introduction to the course outline.
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Lecture:
Experiment protocols, including vessel normalisation using the wire myograph and standard start.
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Lecture:
Pharmacological studies and experimental design (time controls and use of multiple myographs).
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Lecture:
Physiological studies and experimental design (investigating endothelial function, myogenic tone). |
Lecture:
Further myograph applications (culture myography, morphology, histology, biochemistry) and use of other vessels or muscle types. |
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Orientation:
DMT facilities. |
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Lecture:
Maintenance of wire and pressure myograph systems. |
Lecture:
Maintenance of wire and pressure myograph systems. |
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| Coffee/tea break |
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Lecture:
Myography principles: Function and calibration of pressure and wire transducer types. |
Lecture:
Data analysis. |
Demonstration:
Dissection of cerebral and coronary arteries. |
Demonstration:
Removal of endothelium and/or mounting on confocal wire myograph. |
Exercises: Dissection and mounting of arteries in either the wire or pressure myographs. |
| Lunch |
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Afternoon programme |
Introduction: Myograph components and further equipment. |
Exercises: Mesenteric artery dissection and mounting in wire myograph, practice of normalisation and standard start. |
Exercises:
Dissection and mounting of arteries in pressure myograph, construction of pressure-diameter curves. |
Exercises: Dissection and mounting of arteries in either the wire or pressure myographs. |
Demonstration: Culture myograph system and organ bath system. |
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Exercises: Mesenteric artery dissection and mounting in wire myograph. |
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Guest Lecture: Combining myography with fluorescence measurement or electrophysiology. |
Guest Lecture:
To be defined. |
Lecture:
Pitfalls and troubleshooting: where to get help. |
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Evening |
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Group dinner in downtown Århus |
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Optional:
Extra opportunity to focus on wire and pressure systems.
Evaluation and closing.
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