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An isolated organ bath assay is the classical pharmacological screening tool to access concentration-response relationships in contractile tissue. Although a variety of molecular tools have become available in recent decades to assess cellular responses in high-throughput quantities, the organ bath assay is still considered a valuable tool for lead optimization and for the elucidation of the mechanism of action. In addition, organ bath assays are widely used in preclinical safety studies.
The most common application of the organ bath assay is in cardiovascular research, using isolated aorta rings, heart tissue (papillary muscle, left ventricles) or arteries. When studying gastrointestinal effects, preparations of ileum and colon are often used, but also gastric antral muscle and sphincter may be studied.
| DMT Tissue Organ Bath System |
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The multi channel organ bath system is the hallmark of the drive for productivity and results in academic and industry research labs.
The DMT version expand on the classic design with extensive automation of fluid control, programmable washing routines, integrated data aquisition and being GLP capable. All these features increase research throughput, reproducibility in a more hands off approach so laboratory personnel can focus on data analysis rather than washing glassware.
Applications are numerous thanks to a range programmable force transducer, from small blood vessels to large muscle strips or rings from bladder lung or GI system.
Add on options include custom shaped stimulation electrodes and current stimulators to match your tissue. |
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Respiratory effects can be studied in isolated tracheal rings, phrenic diaphragm preparations, pulmonary arterial smooth muscle and even lung parenchyma. Other smooth muscle preparations that are used in organ bath research are urinary bladder, penile muscle strips and prostate.
In contrast to molecular assays, the organ bath assay permits complex responses to be studied where tissue responses are controlled by several physiological parameters. Additionally, the effects of compounds with unknown molecular targets can be studied. Important tissue-specific expression and modification of molecular targets may be lost in cell culture assays but can be studied in the organ bath. Organ baths are also ideal for studying responses in specific animal disease models.
DMT Tissue organ bath system
The workhorse you will become addicted to for your routine, high- throughput pharmacological studies on nearly all type of muscles.
All Tissue organ baths
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