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The Pressure myograph is a system used to study the structure and function of isolated sections of small vessels (diameter >60 μm) under near physiological conditions. Vessel diameter can be measured as a function of pharmacological and physiological stimuli. The vessel wall thickness and lumen dimensions are particularly important factors in small vessel anatomy and pathology. The 111P system includes a DMT microscope, software and hardware for continuous video measurement of vessel diameter and control of intravascular pressure.
«allows measurements of the internal and external diameter of the tissue«
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Features & Benefits |
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High-quality precision research instrument, reliable and easy to control
Study the structure and function of small vessels >60μm under near physiological conditions
Ready to use for luminal flow-studies
Study the effects of pressure and flow
The segments are viable for more than 24 hours
Calibrated video measurements of external and internal diameters within a userdefined area
Continuous measurements of longitudinal force in the segment allow normalization and improve integrity and reproducibility |
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Continuous video measurements of external and internal diameters are done through a window in the base of the myograph. Continuous display of the acquired vessel image can be combined with measurement of vessel dimensions, intravascular pressure, longitudinal force, temperature, luminal flow (optional) and wall calcium concentration (optional). Intravascular pressure or a pressure gradient (user defined to induce flow) over the vessel can be controlled precisely.
A heating system with electronic feedback maintains the chamber temperature eliminating the need for continuous superfusion. The chamber cover includes ports for superfusion, for rapid draining and filling, cumulative addition of drugs and for oxygenation. To facilitate cleaning, the chamber is made of stainless steel and is acid-resistant. All vital measurements are continuously recorded by a computer and are also available as analog outputs for use with a chart recorder or other data acquisition system.
As an option, the FlowMeter - 161FM can be connected in order to measure luminal flow between 15 μl and 1500 μl per minute.
The Myograph & Pressure package
Myograph chamber, interface unit, chamber cover, calibration kit and vacuum valve.
Pressure regulator, calibration kit, internal tubing, set of polished cannulas.
The Acquisition & Analysis package 111P
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Dr. K. Dora, University of Bath, United Kingdom |
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